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Objective:To study the predictive value of combining ultrasound elestography with serological examination on incidences of microvascular invasion (MVI) in patients with hepatocellular carcinoma (HCC).Methods:The clinical data of 288 patients with HCC who underwent liver resection at MengChao Hepatobiliary Hospital of Fujian Medical University from January 2018 to September 2020 were retrospectively analyzed. 104 MVI-negative and 184 MVI-positive patients who were confirmed by postoperative histopathology were divided into the MVI-negative and MVI-positive groups respectively. Serological indicators of alanine aminotransferase, aspartate aminotransferase, platelet, albumin, and alpha-fetoprotein were compared between groups. Imaging indexes including elasticity at liver tumor surrounding 1 cm area (S1), elasticity at liver tumor surrounding 2 cm area (S2), S1S2index (S1/S2×10) and longest tumor diameter were compared between groups. Multi-variate analysis was used to screen out independent risk factors in predicting MVI of hepatocellular carcinoma, and then a nomogram model was constructed.Results:Of 288 patients with HCC who met the inclusion criteria of this study, there were 225 males and 63 females, aged (56.3±9.7) years. Multivariate logistic regression analysis revealed that patients with HCC who had multiple tumors ( OR=2.47, 95% CI: 1.41-4.33, P=0.002), long tumor diameter ( OR=1.21, 95% CI: 1.08-1.36, P=0.031), AFP>400 μg/L ( OR=2.83, 95% CI: 1.54-5.22, P=0.015), a high S1S2index ( OR=1.33, 95% CI: 1.17-1.51, P=0.025) had high incidences of MVI. The nomogram model constructed from these risk factors showed the risk of MVI in HCC patients with a mean absolute deviation of compliance between the predicted value and the true value being 0.021. The receiver operating characteristic (ROC) curve showed that the area under ROC curve of the nomogram model which predicted MVI of HCC patients was 0.777 (95% CI: 0.720-0.835). Conclusions:Multiple tumors, long tumor diameter, AFP>400 μg/L and a high S1S2 index were independent risk factors for MVI in HCC patients. The nomogram model established by these factors accurately predicted the risk of MVI and provided a reference for better choice of treatment.
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Objective@#To preliminary analysis of the characteristics of lymphocyte subsets in peripheral blood among 135 cases of coal worker’s pneumoconiosis patients in Huainan mining area.@*Methods@#The peripheral bloods of 135 cases of coal worker’s pneumoconiosis patients and 112 cases of health examiners were collected. Flow cytometry was used to detect peripheral blood lymphocytes, T cell subsets and CD4+CD25+ regulatory T cells.@*Results@#Compared with the control group, CD64 index of granulocytes and lymphocytes was slightly higher. The total T cells (CD3+) increased in peripheral blood, CD4+ expression was reduced and CD8+ expression was increased in infection group, CD4+/CD8+ ratio was inverted, the differences between the infection group and the control group were statistically significant (P<0.05) . There were significantly fewer NK (nature killer) and B cells, significantly more double negative T cells (DNT, CD3+CD4-CD8-) than the control group (P<0.05) . There was no statistically significant difference of CD4+CD25+CD127+low、CD4+CD25+CD127+hi and the ration of Treg/CD4+CD25+CD127+hi protein expression in peripheral blood in two groups (P>0.05) .@*Conclusion@#The analysis of peripheral blood lymphocyte and subgroup is an ideal index to monitor the immune status of coal worker's pneumoconiosis patients. It has theoretical significance for studying the immune mechanism of pneumoconiosis and guiding clinical treatment.
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Objective To explore renal clear cell carcinoma ( CCRCC) expression and significance of CD146 mR-NA in tumor tissue of patients with. Methods ELISA method for quantitative detection was used for CD146 protein and real-time PCR technique for the detection of CCRCC in 102 ( CCRCC) expression in tumor tissue of patients with CD146 mRNA, and 51 cases of renal patients with non tumor tissues as control. Results Found metastasis in patients with CCRCC, the CD146 protein concentration was statistically significant compared with the control group (F=52.1, P<0.01),the average expression of CD146 mRNA value (0.043 8±0.002 4) was significantly high-er than that of in situ CCRCC patients (0.038 2±0.001 1, P= 0.018) and control group (0.034 4±0.001 0, P=0.001 ) . Conclusion Pathological grading and lymph node up regulation of CD146 mRNA expression in renal cell carcinoma and metastasis, is expected to become a new index, evaluation of malignant degree of CCRCC me-tastasis and prognosis, and provide a reliable basis for the intervention of clinical treatment.
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Objective To investigate the clinical significance of the joint detection of platelet parameters and coagulation function markers in hepatitis patients.Methods 310 patients with viral hepatitis were divided into 4 groups:acute hepatitis group,chronic hepatitis group,serious hepatitis group and hepatocirrhosis group.The control group was the 80 healthy examination eligible people.Each group were detected platelet parameters ( PLT), mean platelet volume( MPV),.platelet distribution width( PDW),platelet large cell ratio( P-LCR), prothrombin time( PT) ,activated partial thromboplasin time (APTT ) , thrombin time ( TT) and factorl ( Fbg).Results The levels of PLT,MPV and Fbg were obviously reduced in chronic hepatitis,serious condition chronic hepatitis patients and hepatocirrhosis had significant difference compared with those in the control group (all P < 0.01).Moreover, the levels of PDW,P-LCR,PT,APTT and TT were obviously increased in the four groups compared with the control group(all P <0.05 ).Conclusion The joint detection of platelet parameters and coagulation function markers could help to observe the degree of progression of virus hepatitis,and had important clinical significance for the treatment and prognosis.